Multiple Length Rings of ϕX174 and S13 Replicative Forms
نویسندگان
چکیده
منابع مشابه
Fragmentation of bacteriophage S13 replicative form DNA by restriction endonucleases from Hemophilus influenzae and Hemophilus aegyptius.
The restriction enzymes Hind from Hemophilus influenzae and Hae,,, from Hemophilus aegyptius cleave bacteriophage S13 replicative form (RF) DNA into 13 and 10 specific fragments, respectively. The sizes of these fragments were estimated by gel efectrophoresis, electron microscopy, and pyrimidine isostich analysis. The Hind and Hae,,, fragments were ordered relative to each other by cross digest...
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The deoxyribonucleic acid (DNA) of bacteriophage S13 was shown to be single-stranded by the criteria of reactivity with formaldehyde, dependence of optical density on ionic strength, broad temperature-absorbance profile, and lack of molar equivalence of the purine and pyrimidine bases. The DNA has a molecular weight of 1.8 x 10(6) daltons, an S degrees (20) of 24.6 in SSC (0.15 m NaCl plus 0.01...
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Trypanosoma cruzi is the etiological agent of Chagas disease. The parasite has to overcome oxidative damage by ROS/RNS all along its life cycle to survive and to establish a chronic infection. We propose that T. cruzi is able to survive, among other mechanisms of detoxification, by repair of its damaged DNA through activation of the DNA base excision repair (BER) pathway. BER is highly conserve...
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When human fibroblasts from different donors are grown in vitro, only a small fraction of the variation in their finite replicative capacity is explained by the chronological age of the donor. Because we had previously shown that telomeres, the terminal guanine-rich sequences of chromosomes, shorten throughout the life-span of cultured cells, we wished to determine whether variation in initial ...
متن کاملThe Mitochondrial S13
The sequence of a wheat mitochondrial reading frame encoding a protein homologous to the E.coli S13 small subunit ribosomal protein has been determined. The gene is located immediately downstream of a 1.4 kb recombinationally-active repeat element that contains the ATPase subunit 6 gene. The coding regions of the two genes are separated by only 153 bp, the shortest distance yet observed between...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1968
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)93192-3